Biotechnology in Forensic Science: Advancements and Applications

Main Article Content

Sunny Antil
Vandana Joon

Abstract

Background: Biotechnology is a multidisciplinary field based on the expertise of molecular biology, chemistry, biochemistry, chemical and biological engineering, and digital computing. Biotechnology plays an important role in modern forensic science, driving advances in analytical tools and techniques.


This review study provides a brief overview of applications, highlighting advances in forensic biotechnology and key technologies involved in the domains of genomics and DNA analysis, microbial forensics, forensic medicine, and forensic serology. The integration of forensic expertise with technology has increased the accuracy, sensitivity, and efficiency of forensic casework.


Conclusion: This interdisciplinary field extends beyond its usual association with biology to also include chemistry, fingerprint analysis, and toxicology, among others. Continued progress and innovation in this advanced field will further enhance investigative capabilities and facilitate the pursuit of justice.

Article Details

Antil, S., & Joon, V. (2025). Biotechnology in Forensic Science: Advancements and Applications. Journal of Forensic Science and Research, 9(1), 007–014. https://doi.org/10.29328/journal.jfsr.1001073
Review Articles

Copyright (c) 2025 Antil S, et al.

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This work is licensed under a Creative Commons Attribution 4.0 International License.

Singh KR. A review paper on the techniques of biotechnology. Int J Emerg Technol Innov Res. 2018;5(9):233-238. Available from: http://www.jetir.org/papers/JETIRFH06040.pdf

Verma AS, Agrahari S, Rastogi S, Singh A. Biotechnology in the realm of history. J Pharm Bioallied Sci. 2011;3(3):321-323. Available from: https://doi.org/10.4103/0975-7406.84430

Agrawal M, Biswas S, Vliet K. Vacuum technology-assisted forensic biotechnology revealing latent fingerprints from fabrics. Vac Technol Coat. 2016;17:32-34. Available from: https://www.researchgate.net/publication/308314408_Vacuum_Technology_Assisted_Forensic_Biotechnology_Revealing_Latent_Finger_Prints_from_Fabrics

Maras MH, Miranda M. Forensic science. In: Encyclopedia of Criminology and Criminal Justice. 2014;1-11. Available from: https://doi.org/10.1080/23779497.2017.1281088

Shukla RK. Forensic biotechnology: Application of flow cytometry in legal medicine. Int J Forensic Sci. 2016. Available from: https://doi.org/10.23880/IJFSC-16000103

Nizami BN, Kazmi SZH, Abid F, Babar MM, Noor A, Zaidi NUS, et al. Omics approaches in forensic biotechnology: looking for ancestry to offence. In: Barh D, Azevedo V, editors. Omics Technologies and Bio-Engineering. Academic Press. 2018:111-129. Available from: https://doi.org/10.1016/B978-0-12-804659-3.00006-3

Bartelink EJ, Chesson LA. Recent applications of isotope analysis to forensic anthropology. Forensic Sci Res. 2019;4(1):29-44. Available from: https://doi.org/10.1080/20961790.2018.1549527

Lewis M, Lainé K, Dawnay L, Lamont D, Scott K, Mariani S, et al. The forensic potential of environmental DNA (eDNA) in freshwater wildlife crime investigations: From research to application. Sci Justice. 2024;64(4):443-454. Available from: https://doi.org/10.1016/j.scijus.2024.06.003

National Forensic Sciences University. Master's and specialization programs in forensic biotechnology. National Forensic Sciences University. [Accessed February 26, 2024]. Available from: https://www.nfsu.ac.in/Programs/programinfo/13?deptid=43

Barkham T. BioRobot EZ1 workstation compares well with manual spin kits for extraction of viral RNA from sera and saves substantial staff time. J Clin Microbiol. 2006;44(4):1598. Available from: https://doi.org/10.1128/JCM.44.4.1598.2006

National Institute of Justice. Automation of sexual assault DNA processing increases efficiency. National Institute of Justice. [Accessed February 26, 2024]. Available from: https://nij.ojp.gov/topics/articles/automation-sexual-assault-dna-processing-increases-efficiency

QIAGEN. QIAsymphony SP/AS. [Accessed February 26, 2024]. Available from: https://www.qiagen.com/us/products/automation/dna-rna-purification/qiasymphony-spas/

Kaunitz JD. The discovery of PCR: ProCuRement of divine power. Dig Dis Sci. 2015;60(8):2230-2231. Available from: https://doi.org/10.1007/s10620-015-3747-0

Pillay VV, Menezes RG, Krishnaprasad R, Pillay M, Lobo SW, Adhikari D, et al. Biotechnology in forensic science: the revolution continues. Nepal Med Coll J. 2007;9(1):57-62. Available from: https://pubmed.ncbi.nlm.nih.gov/17593681/

Shrivastava P, Jain T, Kumawat RK. Direct PCR amplification from saliva sample using non-direct multiplex STR kits for forensic DNA typing. Sci Rep. 2021;11(1):7112. Available from: https://doi.org/10.1038/s41598-021-86633-0

Imaizumi K, Noguchi K, Shiraishi T, Sekiguchi K, Senju H, Fujii K, et al. DNA typing of bone specimens—the potential use of the profiler test as a tool for bone identification. Leg Med (Tokyo). 2005;7(1):31-41. Available from: https://doi.org/10.1016/j.legalmed.2004.07.003

Pereira R, Oliveira J, Sousa M. Bioinformatics and computational tools for next-generation sequencing analysis in clinical genetics. J Clin Med. 2020;9(1):132. Available from: https://doi.org/10.3390/jcm9010132

Jager AC, Alvarez ML, Davis CP, Guzmán E, Han Y, Way L, et al. Developmental validation of the miSeq FGx forensic genomics system for targeted next-generation sequencing in forensic DNA casework and database laboratories. Forensic Sci Int Genet. 2017;28:52-70. Available from: https://doi.org/10.1016/j.fsigen.2017.01.011

Martin P, de Simón LF, Luque G, Farfán MJ, Alonso A. Improving DNA data exchange: Validation studies on a single 6 dye STR kit with 24 loci. Forensic Sci Int Genet. 2014;13:68-78. Available from: https://doi.org/10.1016/j.fsigen.2014.07.002

Oostdik K, Lenz K, Nye J, Schelling K, Yet D, Bruski S, et al. Developmental validation of the PowerPlex® Y23 System: A male-specific, Y-STR multiplex system. Forensic Sci Int Genet. 2014;12:77-88. Available from: https://doi.org/10.1016/j.fsigen.2014.04.013

Whitaker JP, Clayton TM, Urquhart AJ, Millican ES, Downes TJ, Kimpton CP, et al. Short tandem repeat typing of bodies from a mass disaster: high success rate and characteristic amplification patterns in highly degraded samples. Biotechniques. 1995 Apr;18(4):670-7. Available from: https://pubmed.ncbi.nlm.nih.gov/7598902/

Budowle B, Bieber FR, Eisenberg AJ. Forensic aspects of mass disasters: Strategic considerations for DNA-based human identification. Leg Med (Tokyo). 2005;7:230-243. Available from: https://doi.org/10.1016/j.legalmed.2005.01.001

Hughes-Stamm SR, Ashton KJ, van Daal A. Assessment of DNA degradation and the genotyping success of highly degraded samples. Int J Legal Med. 2011;125:341-348. Available from: https://doi.org/10.1007/s00414-010-0455-3

Alvarez-Cubero MJ, Saiz M, Martínez-García B, Sayalero SM, Entrala C, Lorente JA, et al. Next-generation sequencing: An application in forensic sciences? Ann Hum Biol. 2017;44(7):581-592. Available from: https://doi.org/10.1080/03014460.2017.1375155

Haddrill PR. Developments in forensic DNA analysis. Emerg Top Life Sci. 2021;5(3):381-393. Available from: https://doi.org/10.1042/ETLS20200304

Knijff P. From next-generation sequencing to now-generation sequencing in forensics. Forensic Sci Int Genet. 2019;38:175-180. Available from: https://doi.org/10.1016/j.fsigen.2018.10.017

Davenport L, Devesse L, Syndercombe Court D, Ballard D. Forensic identity SNPs: Characterisation of flanking region variation using massively parallel sequencing. Forensic Sci Int Genet. 2023;64:102847. Available from: https://doi.org/10.1016/j.fsigen.2023.102847

Novroski NMM, Cihlar JC. Evolution of single-nucleotide polymorphism use in forensic genetics. Forensic Sci Int Genet. 2022;4:6. Available from: https://doi.org/10.1002/wfs2.1459

Pontes L, Sousa JC, Medeiros R. SNPs and STRs in forensic medicine: A strategy for kinship evaluation. Arch Med Sadowej Kryminol. 2017;67(3):226-240. Available from: https://doi.org/10.5114/amsik.2017.73194

Yagasaki K, Mabuchi A, Higashino T, Wong JH, Nishida N, Fujimoto A, et al. Practical forensic use of kinship determination using high-density SNP profiling based on a microarray platform, focusing on low-quantity DNA. Forensic Sci Int Genet. 2022;61:102752. Available from: https://doi.org/10.1016/j.fsigen.2022.102752

Zhang Q, Wang X, Cheng P, Yang S, Li W, Zhou Z, Wang S. Complex kinship analysis with a combination of STRs, SNPs, and indels. Forensic Sci Int Genet. 2022;61:102749. Available from: https://doi.org/10.1016/j.fsigen.2022.102749

Dash HR, Arora M. CRISPR-CasB technology in forensic DNA analysis: Challenges and solutions. Appl Microbiol Biotechnol. 2022;106(12):4367-4374. Available from: https://doi.org/10.1007/s00253-022-12016-8

Barash M, McNevin D, Fedorenko V, Giverts P. Machine learning applications in forensic DNA profiling: A critical review. Forensic Sci Int Genet. 2024;69:102994. Available from: https://doi.org/10.1016/j.fsigen.2023.102994

Al-Hakim R, Putri E, Hidayah H, Pangestu A, Riani S. Current evidence on bioinformatics' role and digital forensics that contribute to forensic science: Upcoming threat. J Ris Rumpun Matematika Ilmu Pengetahuan Alam. 2022;1(1):25-32. Available from: https://doi.org/10.55606/jurrimipa.v1i1.157

Ray PK. Bioinformatics as a modern tool in forensic science for data understanding & investigation in research. J Forensic Sci Res. 2022;6:83-87. Available from: https://doi.org/10.29328/journal.jfsr.1001040

Wallace H, Jackson AR, Gruber J, Thibedeau AD. Forensic DNA databases: Ethical and legal standards: A global review. Egypt J Forensic Sci. 2014;4:57-63. Available from: https://doi.org/10.1016/j.ejfs.2014.04.002

Behera C, Singh P, Shukla P, Bharti DR, Kaushik R, Sharma N, et al. Development of the first DNA database and identification portal for identification of unidentified bodies in India - UMID. Sci Justice. 2022;62(1):110-116. Available from: https://doi.org/10.1016/j.scijus.2021.12.002

Chunkul S, Sathirapatya T, Dangklao P, Kawicha P, Tammachote R, Vongpaisarnsin K. Enhancing the forensic sexual assault investigations with LAMP-based male DNA detection. Forensic Sci Int Synergy. 2024;10:100567. Available from: https://doi.org/10.1016/j.fsisyn.2024.100567

Guo X, Gu L, Luo Y, Wang S, Luo H, Song F. A bibliometric analysis of microbial forensics from 1984 to 2022: Progress and research trends. Front Microbiol. 2023;14:1186372. Available from: https://doi.org/10.3389/fmicb.2023.1186372

Oliveira M, Amorim A. Microbial forensics: new breakthroughs and future prospects. Appl Microbiol Biotechnol. 2018;102(24):10377-10391. Available from: https://doi.org/10.1007/s00253-018-9414-6

Zhang L, Chen F, Zeng Z, Xu M, Sun F, Yang L, et al. Advances in metagenomics and its application in environmental microorganisms. Front Microbiol. 2021;12:766364. Available from: https://doi.org/10.3389/fmicb.2021.766364

Singh B, Publow A. Forensic body fluid identification using microbiome signature attribution. September 2020. 51 pages.

Dobay A, Haas C, Fucile G, Downey N, Morrison HG, Kratzer A, et al. Microbiome-based body fluid identification of samples exposed to indoor conditions. Forensic Sci Int Genet. 2019;40:105-113. Available from: https://doi.org/10.1016/j.fsigen.2019.02.010

Tozzo P, D'Angiolella G, Brun P, Castagliuolo I, Gino S, Caenazzo L. Skin microbiome analysis for forensic human identification: What do we know so far? Microorganisms. 2020;8(6):873. Available from: https://doi.org/10.3390/microorganisms8060873

Cláudia-Ferreira A, Barbosa DJ, Saegeman V, Fernández-Rodríguez A, Dinis-Oliveira RJ, Freitas AR. On Behalf of the Escmid Study Group of Forensic and Post-Mortem Microbiology Esgfor. The Future Is Now: Unraveling the Expanding Potential of Human (Necro) Microbiome in Forensic Investigations. Microorganisms. 2023;11(10):2509. Available from: https://doi.org/10.3390/microorganisms11102509

Nodari R, Arghittu M, Bailo P, Cattaneo C, Creti R, D'Aleo F, et al. ESCMID Study Group of Forensic and Post-Mortem Microbiology (ESGFOR) and the AMCLI Forensic Microbiology Study Group (GLAMIFO). Forensic Microbiology: When, Where and How. Microorganisms. 2024;12(5):988. Available from: https://doi.org/10.3390/microorganisms12050988

Tejaswi KB, Hari Periya EA. Virtopsy (virtual autopsy): A new phase in forensic investigation. J Forensic Dent Sci. 2013;5(2):146-148. Available from: https://pubmed.ncbi.nlm.nih.gov/24255565/

Turner S, Morrison S. Advances in forensic imaging technology for postmortem analysis. J Med Imaging Health Inform. 2016;6(5):1220-1228.

Varnell RM, Stimac GK, Fligner CL. CT diagnosis of toxic brain injury in cyanide poisoning: Considerations for forensic medicine. Am J Neuroradiol. 1987;8(6):1063-1066. Available from: https://pubmed.ncbi.nlm.nih.gov/3120533/

Ikeda T. Estimating age at death based on costal cartilage calcification. Tohoku J Exp Med. 2017;243:237-246. Available from: https://doi.org/10.1620/tjem.243.237

Grabherr S, Baumann P, Minoiu C, Fahrni S, Mangin P. Post-mortem imaging in forensic investigations: Current utility, limitations, and ongoing developments. Res Rep Forensic Med Sci. 2016;6:25-37. Available from: https://doi.org/10.2147/RRFMS.S93974

Gupta NS, Vandana, Rohatgi R, Gupta S. Title of the article. J Forensic Med Toxicol. 2022;39(2):97-104. Medico Legal Society.

Boroumand M, Grassi VM, Castagnola F, De-Giorgio F, D’aloja E, Vetrugno G, et al. Estimation of postmortem interval using top-down HPLC–MS analysis of peptide fragments in vitreous humour: A pilot study. Int J Mass Spectrom. 2022;483:116952. Available from: https://doi.org/10.1016/j.ijms.2022.116952

Zapico SC, Adserias-Garriga J. Postmortem interval estimation: New approaches by the analysis of human tissues and microbial communities’ changes. Forensic Sci. 2022;2:163-174. Available from: https://doi.org/10.3390/forensicsci2010013

Choi KM, Zissler A, Kim E, Ehrenfellner B, Cho E, Lee SI, et al. Postmortem proteomics to discover biomarkers for forensic PMI estimation. Int J Legal Med. 2019;133(3):899-908. Available from: https://doi.org/10.1007/s00414-019-02011-6

Locci E, Stocchero M, Gottardo R, De-Giorgio F, Demontis R, Nioi M, et al. Comparative use of aqueous humour 1H NMR metabolomics and potassium concentration for PMI estimation in an animal model. Int J Legal Med. 2021;135(3):845-852. Available from: https://doi.org/10.1007/s00414-020-02468-w

Williams T, Soni S, White J, Can G, Javan GT. Evaluation of DNA degradation using flow cytometry: Promising tool for postmortem interval determination. Am J Forensic Med Pathol. 2015;36(2):104-110. Available from: https://doi.org/10.1097/PAF.0000000000000146

Toma L, Vignali G, Maffioli E, Tambuzzi S, Giaccari R, Mattarozzi M, et al. Mass spectrometry-based proteomic strategy for ecchymotic skin examination in forensic pathology. Sci Rep. 2023;13(1):6116. Available from: https://doi.org/10.1038/s41598-023-32520-9

Nagana-Gowda GA, Gowda YN, Raftery D. Expanding the limits of human blood metabolite quantitation using NMR spectroscopy. Anal Chem. 2015;87(1):706-715. Available from: https://doi.org/10.1021/ac503651e

Carr S. Forensic science: The future of body fluid identification. Meas Control. 2009;42(10):310-313. Available from: https://doi.org/10.1177/002029400904201004

Van-Steendam K, De Ceuleneer M, Dhaenens M, Van Hoofstat D, Deforce D. Mass spectrometry-based proteomics as a tool to identify biological matrices in forensic science. Int J Legal Med. 2013;127(2):287-298. Available from: https://doi.org/10.1007/s00414-012-0747-x

Jackson S, Frey BS, Bates MN, Swiner DJ, Badu-Tawiah AK. Direct differentiation of whole blood for forensic serology analysis by thread spray mass spectrometry. Analyst. 2020;145(16):5615-5623. Available from: https://doi.org/10.1039/d0an00857e

Muro CK, Doty KC, de Souza Fernandes L, Lednev IK. Forensic body fluid identification and differentiation by Raman spectroscopy. Forensic Chem. 2016;1:31-38. Available from: https://doi.org/10.1016/J.FORC.2016.06.003

Hanson E, Ingold S, Haas C, Ballantyne J. Messenger RNA biomarker signatures for forensic body fluid identification revealed by targeted RNA sequencing. Forensic Sci Int Genet. 2018;34:206-221. Available from: https://doi.org/10.1016/j.fsigen.2018.02.020

Tighe PJ, Ryder RR, Todd I, Fairclough LC. ELISA in the multiplex era: Potentials and pitfalls. Proteomics Clin Appl. 2015;9(3-4):406-422. Available from: https://doi.org/10.1002/prca.201400130

Bazyar H. On the Application of Microfluidic-Based Technologies in Forensics: A Review. Sensors (Basel, Switzerland). 2023;23(13):5856. Available from: https://doi.org/10.3390/s23135856

Medina-Paz F, Kuba B, Kryvorutsky E, Roca G, Zapico SC. Assessment of blood and semen detection and DNA collection from swabs up to three months after deposition on five different cloth materials. Int J Mol Sci. 2024;25(6):3522. Available from: https://doi.org/10.3390/ijms25063522