Review Article

Review of the Efficiency of Ten Different Commercial Kits for Extracting DNA from Soil Mixed Biological Samples

Neha Mundotiya*, Mukesh Choudhary, Saurabh Jaiswal and Umema Ahmad

Published: 19 July, 2023 | Volume 7 - Issue 1 | Pages: 017-024

Soil-mixed bodily fluids are the most common kind of evidence at outdoor crime scenes. This biological evidence contains DNA, which is a key component of forensic science’s ability to prove an accused person’s guilt because it connects the victim and suspect to the crime scene and aids in identifying the offender and victim. The yield of DNA is significantly influenced by factors including temperature, humidity, storage environment, time since deposition, etc. DNA degradation is caused by a variety of microbes, bacteria, humic acid, and other substances present in soil. Nowadays for DNA extraction, a variety of commercial DNA extraction kits was used now. This paper’s objective is to compare the efficiency of ten different commercial kits used to extract mixed DNA samples. It has been observed that samples stored at a low temperature (-20 °C) are the best for soil blood mixture samples. Compared to samples paired with other types of soil (silt, clay, and marshland), sand soil had the largest production of DNA using the QIAmp investigator kit (Qiagen). Blood Miniprep kit extractions were mostly inhibited, the control that amplified confirms that this kit was the worst in terms of DNA extraction potency. The samples with fewer dirt particles had a much greater yield of DNA.

Read Full Article HTML DOI: 10.29328/journal.jfsr.1001045 Cite this Article Read Full Article PDF


Mixed sample; Degradation; Humic Acid; Outdoor crime; Commercial kits


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