Research Article

Rapid and sensitive identification of cow and buffalo species and gender in tissue/meat samples impounded from different spots in Delhi NCR India by Real Time PCR

Naresh Kumar*, Rahul Jain, Seerat, Amit Chauhan and Deepa Verma

Published: 12 January, 2022 | Volume 6 - Issue 1 | Pages: 012-016

The objective of this study was to obtain a fast, accurate and reliable method of species identification of unknown biological samples for forensic applications, especially in illegal trade of animals as well as meat fraud. Meat fraud and adulteration not only affects the market but also increases the risk of religious and ethnic conflicts around the world [1]. In this study, species-specific and gender differentiating Real time PCR technique was employed to analyse 15 meat samples collected from a suspected site. Out of 15 samples collected from suspected site, 54% and 13% samples were of Cow and buffalo origin respectively. All 54% cow samples were of male while one each of buffalo were of male and female origin. Two samples were inconclusive. These findings indicated that species and gender-specific PCR is very sensitive and can be used for forensic species identification and the detection of meat fraud and adulteration.

Read Full Article HTML DOI: 10.29328/journal.jfsr.1001031 Cite this Article Read Full Article PDF


Real time PCR; Adulteration; Species identification; mtDNA; DNA testing; Meat labeling


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