Abstract

Research Article

Ultraviolet fluorescent detection of elevated bilirubin in dried blood serum

Kelly P Kearse*

Published: 23 September, 2022 | Volume 6 - Issue 1 | Pages: 049-052

Increased levels of bilirubin in blood serum may result from numerous physical conditions including hepatitis, cirrhosis, enzyme deficiency, drug reactions, autoimmune disorders and physiological trauma. No presumptive test for high bilirubin levels in blood serum stains currently exists, which could prove useful in the assessment of crime scenes involving victims with one of the above disorders. Here, the use of ultraviolet 365 (UV 365) is described as a simple, nondestructive method for the detection of blood serum containing elevated levels of bilirubin.

Read Full Article HTML DOI: 10.29328/journal.jfsr.1001036 Cite this Article Read Full Article PDF

References

  1. Fevery J. Bilirubin in clinical practice: a review. Liver Int. 2008 May;28(5):592-605. doi: 10.1111/j.1478-3231.2008.01716.x. PMID: 18433389.
  2. Fargo MV, Grogan SP, Saguil A. Evaluation of Jaundice in Adults. Am Fam Physician. 2017 Feb 1;95(3):164-168. PMID: 28145671.
  3. Muchowski KE. Evaluation and treatment of neonatal hyperbilirubinemia. Am Fam Physician. 2014 Jun 1;89(11):873-8. PMID: 25077393.
  4. Gondal B, Aronsohn A. A Systematic Approach to Patients with Jaundice. Semin Intervent Radiol. 2016 Dec;33(4):253-258. doi: 10.1055/s-0036-1592331. PMID: 27904243; PMCID: PMC5088098.
  5. Purohit T, Cappell MS. Primary biliary cirrhosis: Pathophysiology, clinical presentation and therapy. World J Hepatol. 2015 May 8;7(7):926-41. doi: 10.4254/wjh.v7.i7.926. PMID: 25954476; PMCID: PMC4419097.
  6. Kalakonda A, Jenkins BA, John S. Physiology, Bilirubin. [Updated 2021 Sep 16]. In: StatPearls [Internet]. Treasure Island (FL): StatPearls Publishing; 2022. https://www.ncbi.nlm.nih.gov/books/NBK470290/
  7. Hossain MR, Pandey RK, Islam MF, Datar P, Gayam V, Puri P, Malar T. Acute Isolated Hyperbilirubinemia as a Presentation of Alcoholic Liver Disease: A Case Report and Literature Review. Case Reports Hepatol. 2018 Feb 12;2018:9403934. doi: 10.1155/2018/9403934. PMID: 29623226; PMCID: PMC5829357.
  8. Scharf C, Liebchen U, Paal M, Becker-Pennrich A, Irlbeck M, Zoller M, Schroeder I. Successful elimination of bilirubin in critically ill patients with acute liver dysfunction using a cytokine adsorber and albumin dialysis: a pilot study. Sci Rep. 2021 May 13;11(1):10190. doi: 10.1038/s41598-021-89712-4. PMID: 33986443; PMCID: PMC8119427.
  9. Souza MV, Novaes FS. Icterícia grave e miocardiopatia tireotóxica com trombo atrial [Serious jaundice and thyrotoxic myocardiopathy with atrial thrombus]. Arq Bras Endocrinol Metabol. 2012 Oct;56(7):456-60. Portuguese. doi: 10.1590/s0004-27302012000700008. PMID: 23108751.
  10. Ali H, Rizvi T, Niazi M, Galan M, Pyrsopoulos N. Autoimmune Hepatitis Induced after Treatment of Syphilitic Hepatitis. J Clin Transl Hepatol. 2022 Feb 28;10(1):174-177. doi: 10.14218/JCTH.2020.00178. Epub 2021 May 31. PMID: 35233387; PMCID: PMC8845154.
  11. Baig M, Zheng L, Farmer A. Severe Hyperbilirubinemia: A Rare Complication of Lyme Disease. Case Rep Gastrointest Med. 2019 Dec 24;2019:2762389. doi: 10.1155/2019/2762389. PMID: 31956450; PMCID: PMC6949660.
  12. Lin PMF, Chow SCY, Ng SWY, Ho AMH, Wan S. Acute liver failure with extreme hyperbilirubinemia secondary to endocarditis-related severe mitral and tricuspid regurgitation: a challenge and an opportunity for surgeons. J Thorac Dis. 2018 Feb;10(2):1067-1071. doi: 10.21037/jtd.2018.01.138. PMID: 29607182; PMCID: PMC5864625.
  13. Labori KJ, Raeder MG. Diagnostic approach to the patient with jaundice following trauma. Scand J Surg. 2004;93(3):176-83. doi: 10.1177/145749690409300302. PMID: 15544071.
  14. Cambou M, Greeley AM. Clinical Vignette: A case of autoimmune hepatitis. Proc UCLA Healthcare 2015; 19:1-3.
  15. Kearse KP. Ultraviolet 365 as an Alternative Light Source for Detection of Blood Serum. J Forensic Sci. 2020 Sep;65(5):1716-1721. doi: 10.1111/1556-4029.14439. Epub 2020 Apr 28. PMID: 32343369; PMCID: PMC7496641.
  16. Kearse KP. Environmental influence on blood serum detection using ultraviolet 365. J. Forensic Sci Res. 2021; 5: 130-036. doi: 10.29328/journal.jfsr.1001024
  17. Kearse KP. The reddish color of bloodstains on the Shroud of Turin: investigation of two hypotheses. J His Arch & Anthropol Sci. 2020; 5:101‒107. DOI: 10.15406/jhaas.2020.05.00223

Figures:

Figure 1

Figure 1

Figure 1

Figure 2

Figure 1

Figure 3

Figure 1

Figure 4

Figure 1

Figure 5

Figure 1

Figure 6

Similar Articles

Recently Viewed

  • Approaching Mental Health Through a Preventive Data Analysis Platform
    Gabriel F Pestana and Olga Valentim* Gabriel F Pestana, Olga Valentim*. Approaching Mental Health Through a Preventive Data Analysis Platform. Arch Psychiatr Ment Health. 2024: doi: 10.29328/journal.apmh.1001052; 8: 020-027
  • Second Stage of Labor Cesarean Section Maternal and Fetal Outcomes
    Ahazeej Gurashi, Ameer Osman, Hajar Suliman, Ayat Eltigani, Isra Siralkatim, Hamza Orfali and Awadalla Abdelwahid Suliman* Ahazeej Gurashi, Ameer Osman, Hajar Suliman, Ayat Eltigani, Isra Siralkatim, Hamza Orfali, Awadalla Abdelwahid Suliman*. Second Stage of Labor Cesarean Section Maternal and Fetal Outcomes. Clin J Obstet Gynecol. 2024: doi: 10.29328/journal.cjog.1001159; 7: 025-033
  • Surgical and Delivery Outcomes of Coexisting Uterine Fibroids with Pregnancies in Nigeria
    Ade-Ojo Idowu Pius* and Odetola Amoo A Ade-Ojo Idowu Pius*, Odetola Amoo A. Surgical and Delivery Outcomes of Coexisting Uterine Fibroids with Pregnancies in Nigeria. Clin J Obstet Gynecol. 2024: doi: 10.29328/journal.cjog.1001161; 7: 037-041
  • Endometriosis as a risk factor for colorectal cancer
    Víctor Manuel Vargas-Hernández*, José María Tovar- Rodríguez and Víctor Manuel Vargas-Aguilar Víctor Manuel Vargas-Hernández*,José María Tovar- Rodríguez,Víctor Manuel Vargas-Aguilar . Endometriosis as a risk factor for colorectal cancer. Clin J Obstet Gynecol. 2020: doi: 10.29328/journal.cjog.1001057; 3: 093-097
  • Pediatric Dysgerminoma: Unveiling a Rare Ovarian Tumor
    Faten Limaiem*, Khalil Saffar and Ahmed Halouani Faten Limaiem*, Khalil Saffar, Ahmed Halouani. Pediatric Dysgerminoma: Unveiling a Rare Ovarian Tumor. Arch Case Rep. 2024: doi: 10.29328/journal.acr.1001087; 8: 010-013

Read More

Most Viewed

Read More

Help ?